Description
STRAIN-IDENTIFIED CULTURE
Bacillus subtilis D165
Bacillus subtilis D165 is supplied as a strain-identified freeze-dried culture for B2B probiotic blends. The purchasing definition is the strain designation and the delivered potency rather than the species name alone.

COA-Backed Specification
| Identity | Bacillus subtilis |
|---|---|
| Strain designation | D165 |
| Plant part | Freeze-dried culture, strain-identified |
| Form and colour | Fine off-white to cream powder |
| Assay | Not less than 1.0 x 10^11 CFU/g |
| Method | HPLC |
| Particle size | 98% through 80 mesh |
| Loss on drying | <=5.0% |
| Ash | <=5.0% |
| Heavy metals | Within the limits of the approved specification |
| Microbiological limits | Within the limits of the approved specification |
| Representative batch | LXXJ260504 |
| Manufacture date | 4 May 2026 |
| Shelf life | Two years when stored properly |
Representative values do not replace the current shipment COA.

Buyer Decision Guide
Probiotics are specified by strain, potency at delivery and enumeration method. Confirm the strain designation, the CFU basis (per gram or per dose) and the overage required to hold potency through your shelf life and packing format.
- Confirm the strain designation, not only the species
- Check the CFU basis and enumeration method
- Review water activity and viability data
- Confirm cold-chain and packing requirements

How This Grade Differs
D165 is a specific strain and is not interchangeable with other strains of the same species. Potency, water activity and enumeration results differ by strain and by production lot, so qualification must be strain-based.
Compare these related grades before approval:
For a substitution review, compare botanical identity, plant part, assay target, analytical method, physical form and the current offered-batch COA.
Application Areas
- Probiotic capsules and stick packs
- Powdered functional beverages
- Fermented and cultured food projects
- Private-label microbiome products
Packaging and Delivery
- Evaluation sample typically 10-20 g, subject to stock
- Regular MOQ typically 1 kg
- 1 kg foil bag or 25 kg fibre drum with inner food-grade liner
- Custom packing and private-label options subject to confirmation
- Typical lead time 7-15 working days after deposit
Quality and Documentation
Representative batch LXXJ260504 supports the specification table above. Use the cited record for preliminary comparison and request the offered-lot COA for final technical approval. Batch COA, specification sheet, agreed third-party testing and export documents are available for buyer verification.
Frequently Asked Questions
How is lot traceability handled?
Each lot keeps batch records and a retained sample, and the certificate for LXXJ260504 carries the same batch reference used in the quotation.
Is the declared percentage a specification limit or a measured result?
The specification figure is Not less than 1.0 x 10^11 CFU/g; the measured result for your lot is reported separately on its COA.
How long can we hold stock before use?
Two years when stored properly from the manufacture date when the pack stays sealed and dry.
Which particle size or presentation applies?
98% through 80 mesh
What happens to viability during transport?
The declared count is Not less than 1.0 x 10^11 CFU/g; shipping conditions and the receiving test are agreed so the count is verified on arrival.
Can you produce to our own specification or packing?
Alternative assay targets, particle size, packing and private-label requirements are reviewed case by case, subject to production feasibility and a revised written specification.
What packing options are available?
Standard packing is a sealed food-grade inner liner inside an export carton or fibre drum. Pack size, labelling and private-label artwork are agreed in the written specification.
Which documents come with the shipment?
Batch COA, specification sheet, agreed third-party testing where required, commercial invoice, packing list, certificate of origin and transport documents. The exact set is confirmed against the product and destination market.
Request COA, Sample or Quotation
Send your target specification, application, order quantity, destination country and required test limits.
Get the current batch COA, a sample or a quotation




