Description
STRAIN-IDENTIFIED CULTURE
Enterococcus faecalis D258
Enterococcus faecalis D258 is supplied as a strain-identified freeze-dried culture for B2B probiotic blends. The purchasing definition is the strain designation and the delivered potency rather than the species name alone.

COA-Backed Specification
| Identity | Enterococcus faecalis |
|---|---|
| Strain designation | D258 |
| Plant part | Freeze-dried culture, strain-identified |
| Form and colour | Fine off-white to cream powder |
| Assay | Not less than 5.0 x 10^11 CFU/g |
| Method | HPLC |
| Particle size | 98% through 80 mesh |
| Loss on drying | <=5.0% |
| Ash | <=5.0% |
| Heavy metals | Within the limits of the approved specification |
| Microbiological limits | Within the limits of the approved specification |
| Representative batch | LXXJ260309 |
| Manufacture date | 9 Mar 2026 |
| Shelf life | Two years when stored properly |
Representative values do not replace the current shipment COA.

Buyer Decision Guide
Probiotics are specified by strain, potency at delivery and enumeration method. Confirm the strain designation, the CFU basis (per gram or per dose) and the overage required to hold potency through your shelf life and packing format.
- Confirm the strain designation, not only the species
- Check the CFU basis and enumeration method
- Review water activity and viability data
- Confirm cold-chain and packing requirements

How This Grade Differs
D258 is a specific strain and is not interchangeable with other strains of the same species. Potency, water activity and enumeration results differ by strain and by production lot, so qualification must be strain-based.
Compare these related grades before approval:
For a substitution review, compare botanical identity, plant part, assay target, analytical method, physical form and the current offered-batch COA.
Application Areas
- Probiotic capsules and stick packs
- Powdered functional beverages
- Fermented and cultured food projects
- Private-label microbiome products
Packaging and Delivery
- Evaluation sample typically 10-20 g, subject to stock
- Regular MOQ typically 1 kg
- 1 kg foil bag or 25 kg fibre drum with inner food-grade liner
- Custom packing and private-label options subject to confirmation
- Typical lead time 7-15 working days after deposit
Quality and Documentation
Representative batch LXXJ260309 supports the specification table above. Use the cited record for preliminary comparison and request the offered-lot COA for final technical approval. Batch COA, specification sheet, agreed third-party testing and export documents are available for buyer verification.
Frequently Asked Questions
What physical form does Enterococcus faecalis D258 Freeze-Dried Powder ship in?
Fine off-white to cream powder, milled to 98% through 80 mesh.
How is lot traceability handled?
Each lot keeps batch records and a retained sample, and the certificate for LXXJ260309 carries the same batch reference used in the quotation.
What happens to viability during transport?
The declared count is Not less than 5.0 x 10^11 CFU/g; shipping conditions and the receiving test are agreed so the count is verified on arrival.
Which safety parameters are tested every lot?
Moisture <=5.0%, ash <=5.0%, heavy metals and microbiology against the approved specification.
How is Enterococcus faecalis D258 Freeze-Dried Powder quantified?
By HPLC against the specification figure of Not less than 5.0 x 10^11 CFU/g.
Which documents come with the shipment?
Batch COA, specification sheet, agreed third-party testing where required, commercial invoice, packing list, certificate of origin and transport documents. The exact set is confirmed against the product and destination market.
What is the minimum order quantity?
The regular MOQ is typically 1 kg. An evaluation sample of 10-20 g can usually be arranged before a formal order, subject to stock.
Are you a manufacturer or a trading company?
LongBio is Shaanxi Longsheng Biotechnology Co., Ltd., a direct manufacturer of botanical extracts and functional ingredients integrating R&D, production and export support. Factory review can be arranged by video or an on-site visit.
Request COA, Sample or Quotation
Send your target specification, application, order quantity, destination country and required test limits.
Get the current batch COA, a sample or a quotation




