Description
STRAIN-IDENTIFIED CULTURE
Bifidobacterium breve HC2953
Bifidobacterium breve HC2953 is supplied as a strain-identified freeze-dried culture for B2B probiotic blends. The purchasing definition is the strain designation and the delivered potency rather than the species name alone.

COA-Backed Specification
| Identity | Bifidobacterium breve |
|---|---|
| Strain designation | HC2953 |
| Plant part | Freeze-dried culture, strain-identified |
| Form and colour | Fine off-white to cream powder |
| Assay | Not less than 3.0 x 10^11 CFU/g |
| Method | HPLC |
| Particle size | 98% through 80 mesh |
| Loss on drying | <=5.0% |
| Ash | <=5.0% |
| Heavy metals | Within the limits of the approved specification |
| Microbiological limits | Within the limits of the approved specification |
| Representative batch | LXXJ260204 |
| Manufacture date | 4 Feb 2026 |
| Shelf life | Two years when stored properly |
Representative values do not replace the current shipment COA.

Buyer Decision Guide
Probiotics are specified by strain, potency at delivery and enumeration method. Confirm the strain designation, the CFU basis (per gram or per dose) and the overage required to hold potency through your shelf life and packing format.
- Confirm the strain designation, not only the species
- Check the CFU basis and enumeration method
- Review water activity and viability data
- Confirm cold-chain and packing requirements

How This Grade Differs
HC2953 is a specific strain and is not interchangeable with other strains of the same species. Potency, water activity and enumeration results differ by strain and by production lot, so qualification must be strain-based.
Compare these related grades before approval:
- Bifidobacterium bifidum HC2273 Freeze-Dried Powder
- Bifidobacterium adolescentis HC2916 Freeze-Dried Powder
For a substitution review, compare botanical identity, plant part, assay target, analytical method, physical form and the current offered-batch COA.
Application Areas
- Probiotic capsules and stick packs
- Powdered functional beverages
- Fermented and cultured food projects
- Private-label microbiome products
Packaging and Delivery
- Evaluation sample typically 10-20 g, subject to stock
- Regular MOQ typically 1 kg
- 1 kg foil bag or 25 kg fibre drum with inner food-grade liner
- Custom packing and private-label options subject to confirmation
- Typical lead time 7-15 working days after deposit
Quality and Documentation
Representative batch LXXJ260204 supports the specification table above. Use the cited record for preliminary comparison and request the offered-lot COA for final technical approval. Batch COA, specification sheet, agreed third-party testing and export documents are available for buyer verification.
Frequently Asked Questions
How is appearance checked?
Fine off-white to cream powder, compared against a retained sample from the approved batch.
Which batch is the quotation based on?
The page cites representative batch LXXJ260204; the current lot’s certificate governs release.
What happens to viability during transport?
The declared count is Not less than 3.0 x 10^11 CFU/g; shipping conditions and the receiving test are agreed so the count is verified on arrival.
How should the material be stored after arrival?
Two years when stored properly. Keep the pack sealed; moisture uptake is the main risk for this grade.
Is the declared percentage a specification limit or a measured result?
The specification figure is Not less than 3.0 x 10^11 CFU/g; the measured result for your lot is reported separately on its COA.
Which documents come with the shipment?
Batch COA, specification sheet, agreed third-party testing where required, commercial invoice, packing list, certificate of origin and transport documents. The exact set is confirmed against the product and destination market.
Can you produce to our own specification or packing?
Alternative assay targets, particle size, packing and private-label requirements are reviewed case by case, subject to production feasibility and a revised written specification.
Are you a manufacturer or a trading company?
LongBio is Shaanxi Longsheng Biotechnology Co., Ltd., a direct manufacturer of botanical extracts and functional ingredients integrating R&D, production and export support. Factory review can be arranged by video or an on-site visit.
Request COA, Sample or Quotation
Send your target specification, application, order quantity, destination country and required test limits.
Get the current batch COA, a sample or a quotation




