BOTANICAL INGREDIENT BUYER GUIDE
HPLC vs UV vs TLC for Botanical Extract Testing
Why results from HPLC, UV and TLC are not automatically interchangeable, and what buyers should confirm before comparing botanical extract quotations.
Method Comparison
| Item | What it tells the buyer | Why it matters |
|---|---|---|
| HPLC | Separates and quantifies specific compounds | Marker-specific assays and profiles |
| UV | Measures overall absorbance response | Defined validated total-content methods |
| TLC | Provides a qualitative identity fingerprint | Identity screening and comparison |
| Buyer rule | Compare results only with the stated method | Preventing false like-for-like comparisons |
Evidence Boundary
This guide explains purchasing review principles. It does not certify a product or replace the specification and current offered-batch COA for the grade being purchased.
Start with Product Identity
Confirm the botanical or material identity, plant part and physical form. A raw powder, ratio extract and standardised extract should not be treated as equivalent purchasing specifications.
Match the Assay to Its Method
Record the analyte, target, unit and method together. HPLC, UV, TLC and GC answer different analytical questions; a numerical result without its method can be misleading.
Review Batch and Quality Data
Check batch number, manufacture date, appearance, particle size, loss on drying, ash, heavy metals and microbiological limits where applicable. Ask for the current offered-batch COA before final approval.
Compare Commercial Requirements
Confirm sample availability, MOQ, lead time, packing, destination market and required declarations. Qualification is complete only when the technical and commercial offer refer to the same grade.
Questions to Send the Supplier
- What exact botanical source and plant part are offered?
- What assay target and method apply?
- Is the COA representative or shipment-specific?
- Which physical and contaminant limits are controlled?
- Which documents are available for the destination market?